“Orthogonal Replication for Rapid Evolution and Synthetic Biology”
Tuesday, March 28, 2017 - 12:30 to 13:30
Abstract: We recently developed an orthogonal DNA replication (OrthoRep) system in the yeast Saccharomyces cerevisiae. OrthoRep exploits an unusual selfish DNA element consisting of a DNA plasmid–DNA polymerase pair that stably replicates in the cytoplasm of yeast. The spatial and mechanistic isolation of OrthoRep from genomic replication may have fundamental significance for protein engineering and synthetic biology.