In order to mimic biological cells in real world to separate chemical mixtures, we need to understand how they behave. My research proposes a new theory to understand the mechanism of them when very efficient, fast, and a robust separation happens in membranes with nanometer size. Also, my thesis focuses on using carbon nanotube as a nanopore for separating DNA, and optimizing carbon nanotube optical property at single molecular level for future sensing applications.
My overall research interests include understanding the mechanical and dynamic properties of soft and biological materials. Biopolymers, as well as carbon nanotubes can be characterized as semiflexible polymers, which have been shown to exhibit elastic and relaxational properties that differ strongly from flexible polymers. By simulating networks of semiflexible polymers, we can predict their mechanical response, as well as the dynamics of their stress relaxation.
My research focuses on modeling the soft condensed matter and understanding the underlying physics of biological many-body systems. Our goal is to develop both analytical theories and numerical tools that enhance our knowledge about those complex systems. Specifically, I work on modeling cytoskeleton systems utilizing kinetic Monte Carlo simulation and refining nonequilibrium theories on active matters. My research interests also cover glassy dynamics of active matters and the reaction kinetics of molecular motors.
Many bacterial species exhibit self-organization behaviors where individual cells move collectively and organize themselves into a variety of multi-cellular structures. Majority of previous studies focused on biochemical signaling among cells to understand the mechanisms behind the bacterial self-organization. However, mechanical interactions among cells can also play an important role in this self-organization process. My research work focuses on understanding the role of mechanical interactions in various self-organization behaviors observed in Myxococcus xanthus bacteria using biophysical cell models and agent-based computer simulations. I investigate the mechanisms of individual to collective cell motility, collective alignment of cells into groups, aggregation of cells into circular/spiral patterns in M. xanthus.
My current projects include investigations into the following three topics; chemotaxis, intercellular signaling and pattern formation. On the chemotaxis front, I am trying to build a theoretical model to explain early stages of chemotactic aggregation in the slime mold dictyostelium discoideum. This involves modeling at multiple space and time scales. I am also studying how cell-cell contact signaling can lead to tissue behavior and patterning in the context of the Notch signaling pathway and the planar cell polarity (PCP) pathway. The overall goal is to investigate design principles of these cellular mechanisms (physical or biochemical) that lead to specific cellular behaviors (cell fate, pattern formation) using numerical methods and simulations.
My current research interests focus on the formulation of mathematical approaches to model biologically-relevant systems where the interactions between microscopic components (i. e. cells, proteins) play a crucial role in establishing the emerging features of a system. Bacterial biofilms are a fascinating example where cell-cell interaction and communication mechanisms determine cell phenotypic expression at a single cell level and emerging characteristics at the colony level. Another application of current interest is the modeling of the epithelial-to-mesenchimal transition (EMT) that characterizes cancer cells that enter the bloodstream and adhere to tissues in secondary sites, giving rise to metastases.
My research interests focus on how statistical mechanics principles can be used to describe macromolecular dynamics in an effective way. Specifically, I have worked on developing methods for designing suitable collective coordinates out of complex data. Currently, I am investigating new data-based strategies to formulate a rigorous theoretical framework for the systematic coarse graining of complex macromolecules.
Currently, I am working on a comprehensive literature review comprising fundamental, applied, and prospective research on the CRISPR (clustered regularly interspaced short palindromic repeats) genetic adaptive immune system of prokaryotes. I have also been conducting background research that unifies principles from physics and neuroimaging to develop a theoretical framework for understanding multimodal integration in the human brain.
The goal of my work is to improve the models used to predict the stability of proteins. I use several methods including direct coupling analysis, the AWSEM forcefield and normal mode analysis. The insights obtained by these analyses can also be used to analyze the movement of the protein during allosteric regulation.
I develop tools (software, hardware, and wetware) to introduce precise time-varying perturbations into bacterial gene networks using optogenetics. I am applying these methods to study the network responsible for spore differentiation in B. subtilis.
Coarse-grained (CG) models are an attractive way to reduce computation time as protein sizes grow larger. My work focuses on the definition of a theoretical framework that incorporates experimental data (e.g. FRET efficiencies, free energy differences, NMR measurements) into the definition of an effective CG force-field that can correctly reproduce the observed results.
My current research majorly focuses on protein aggregation and its role in the formation of long-term memory and neurodegenerative diseases using energy landscape theory. For aggregation in the formation of long-term memory, we proposed the idea of the mechanical prion (CPEB) that couples aggregation and cytoskeleton dynamics in the synapse. We are still working on the molecular details of the coupling, as well as understanding the formation of LTP from a systems biology level. For aggregation in neurodegenerative diseases, we detailed the aggregation free energy landscapes of polyglutamine repeats and Amyloid-beta proteins.
I am interested in investigating the mechanism, relationship between the protein structure and its property. At this time, I am working on the development of protein prediction based on our coarse grain model (AWSEM).
My work focuses on modeling the cell-fate decision in bacteriophage lambda. My goal is to quantitatively describe the gene expression kinetics of the lambda decision and to identify and characterize the main factors that drive it.
I am interested in the mechanistic processes by which viruses infect their host and replicate, and more generally in lipid dynamics and fusion. The spread of length and timescales involved in various aspects of the viral life cycle necessitates a blend of modeling approaches, ranging from very coarse-grained simulations to detailed atomistic models. I primarily use molecular dynamics simulations to study conformational transitions in proteins, with the eventual goal of coupling these functional motions in viral systems to the lipid dynamics themselves for a complete structural picture of viral infection. Understanding this problem provides not only general insights about bilayer fusion, important for many cellular processes, but also has a very practical upside in potentially aiding therapeutic development.
We seek to study the physics of living systems. Specifically, I study the theory of protein folding in crowded environments such as inside a cell. Secondly, I am highly interested in understating the evolvement of cytoskeletal networks in order to study the long-term formation of memory. In both endeavors we use high performance computing resources and the theory of computational science to explain the physical phenomena.
My project seeks to understand the mechanism of encoding calmodulin states via Ca 2+ signals, and its downstream interaction with the multiple states of calmodulin-dependent kinase II. Our expected result will provide a tool for predicting protein-protein interaction that extends beyond the CaM/CaMKII examples.
Our goal is to understand protein folding and protein dynamics in vivo, using a combined approach of theory and computer simulations. Inside a cell, protein folding occurs in a highly crowded environment, where volume exclusion from surrounding macromolecules affects the dynamics and conformation space. Density fluctuation of these macromolecules creates a void where the protein resides that statistically favors a compact conformation over an extended one. Furthermore, new folded states appear in the presence of macromolecular crowding. We have modified our coarse-grained molecular dynamics simulations to account for pressure, and provide molecular insight to experiments.
My general research interest involves mathematical modeling of mechanisms of drug resistance in cancer. Currently, I study the immune-cancer dynamics in the setting of T cell immunotherapy, as well as inferential statistical models of partial epithelial-mesenchymal transition (EMT) signatures.
Simulations are playing a significant role in advances of different areas of science. While every simulation framework supports different platforms to be executed on, they need constant modification in order to adapt to new architectures. As a result, supporting upcoming architectures is time-consuming and error-prone. To address this issue, new programming models are introduced that are platform agnostic and make simulation code portable on different architectures. However, they cannot reach performance of target-specific implementations. In a nutshell, portability leads to performance degradation. While we make our code more portable, we lose performance. My research tries to address this trade-off.
The purpose of my work is to study models of multiple particle systems. We are trying to analyze theoretical models and Monte Carlo simulations as a way of describing many particles behavior in a one-dimension lattice.
ALEXANDRU DAN GRIGORE
My project revolves around an aggressive subtype of prostate cancer known as neuroendocrine differentiation. I am currently assessing whether these cancer cells display a true neuronal phenotype, which might better explain the resistance to treatment and poor prognosis. Next, I will assess whether these cancer cells communicate via action potential-like impulses and/or synapse-like connections. If such true neuronal signaling does exist, I will then try to block it at various levels by using pharmacological agents that are currently being used for neurological and/or psychiatric diseases. This might ultimately lead to new therapeutic approaches for aggressive prostate cancer.
My research focuses on the analysis of macromolecular dynamics and the development of new strategies for enhanced sampling. Current methods for adaptive sampling are mostly based on dimensionality reduction tools, but the speed-up achieved for complex systems is still limited. Analyzing the shortcomings of the current methods allows us to improve these methods and develop new approaches, in order to better simulate and characterize protein dynamics over long timescales.
My research work focuses on uncovering the operating principles underlying Epithelial-to-Mesenchymal Transition (EMT) and cancer metabolism. Using the network modeling approach, we identified a hybrid Epithelial/Mesenchymal (E/M) phenotype during EMT and several 'phenotypic stability factors', such as OVOL and GRHL2, which can stabilize the hybrid E/M phenotype. Both predictions have been verified experimentally. Currently I am working on modeling the interplay between glycolysis and oxidative phosphorylation in cancer. The long-term goal is to understand the connection between EMT and metabolism during cancer progression.
My research mainly focuses on cancer system biology. Transitions between epithelial and mesenchymal phenotypes play important roles in both tissue repair and cancer metastasis. Currently, I’m investigating the influences of new feedback terms on EMT and MET. Some would have significant effects on the transitions and need to be further studied.
My research investigates how the essential physics regulating macromolecular dynamics and function can be captured in coarse-grained models. I am exploring new strategies to design coarse-grain models by considering new functional forms and by incorporating experimental measurements in the simulation.
My research interests include computational modeling and analysis of biomolecular dynamics and applications of machine learning. I am particularly interested in coarse-grained network modeling and the interplay of flexibility and frustration in the large scale conformational changes that accompany biomolecular functions.
Protein search for their binding sites on DNA is an essential biological phenomenon crucial for the most biological processes. Proteins have to find and recognize their specific targets fast and precise in spite of the huge length of DNA and small size of target as well as a complicated nature of protein-DNA interactions in vivo. Although, significant experimental and theoretical efforts were made in recent years, the mechanism of this process remains not well understood and many aspects are still uncovered. Our goal is to create mathematical framework, capturing the most relevant physical-chemical processes that are consistent with the experiments in all range of parameters and gives correct prediction in the limits.
I am currently working on understanding long-term morphological plasticity of dendritic spines by computationally integrating known short-term biochemical signals and actin cytoskeleton reorganization. This study, when successful, will considerably advance our understanding of cellular mechanism of learning and memory.
I am interested in investigating the physical mechanism underlying the functions of various biological molecules. Specifically, I have been using different tools from physics and chemistry to understand the dynamical picture of how influenza viruses invade their host cells. I am also working on the development of coarse-grained models for protein structure predictions.
Membrane proteins account for more than 20 percent of all human protein-coding genes and more than 50 percent of the drug targets using today. My current research focuses on the studying of force induced membrane protein unfolding dynamics using our coarse grain model (AWSEM).
My current research includes studies of ERK and kinesin motor proteins. I use both theoretical methods and computational tools (such as molecular dynamics, docking, etc.) to understand their properties and their role in the living cells.
I am interested in the elastic properties of semi-flexible polymer networks. In living systems, such networks provide striking nonlinear mechanical behavior to cells and tissues, including strain stiffening and negative normal stresses. Currently, I am working to characterize the dependence of negative normal stress on network structure and applied strain.
LAN HOA TRINH
My current study mainly focuses on understanding the problem of protein folding in the cell-like conditions by using coarse-grained models, computer simulations and statistical physics approaches.
I am interested in studying the dynamics of evolution in heterogeneous and changing environments, using stochastic simulations, physical principles, and mathematical models. Of particular interest are the emergence of antibiotic resistance in bacteria and the emergence of drug resistance in cancer cells. I am also interested in understanding the general theoretical principles governing biological evolution and how these can be applied in a clinical setting, for example, in cancer prognosis.
My research focuses on the effect of modularity on biological evolution. Currently I am studying how modularity coupled with horizontal gene transfer accelerates the migration of human beings and bacteria. The insights from my studies could also be used in the study of cancer metastasis.
My main focus is the quantification of the stochastic kinetics of transcription at a single gene locus in E. coli.
My research concerns multiscale modeling of gene regulatory networks, using ideas and knowledges from dynamical systems, statistical mechanics and kinetic theory of physical chemistry. I am also interested in analyzing genomics data using machine learning techniques.
I am interested in the collective behaviors of cells. Specifically, I have been investigating the mechanisms for the formation of finger-like instabilities arising from the tissue border in collective migrating cell sheets.
I am a graduate student in the department of Biochemistry and Molecular Biology at Baylor College of Medicine, working in the lab of Dr. Ido Golding. I am working on the system comprising the bacterium E. coli and phage lambda. My goal is to discover the undetected variables that contribute to the heterogeneity of lambda gene expression and the resulting decision between lysis and lysogeny.
My research mainly focuses on understanding human cognitive process and psychiatric diseases (e.g. major depression). Data is acquired from resting-state fMRI and both computational and theoretical approaches are adopted.
I study the durotaxis and chemotaxis. For durotaxis, I got and solved Fokker–Planck equation. I also utilized Monte Carlo Simulation to calculate the variance of persistent random walk. I also simulated the trajectory of single cell in uniform substrate and gradient substrate. For chemotaxis, I studied the advantage of exosomes. I studied the stability and instability of coupled partial differential equation by simulation. I studied the influence of exosome lifetime to the attraction. This theory maybe used to explain why some immune neurotransmitters are transported in exosomes.
I study protein folding under conditions that are present in the interior of a cell. My current interest is to understand how hydrodynamics interaction and the macromolecular crowding effect affects the folding of proteins.
My main focus is the quantification of transcription kinetics at the level of individual gene copies in live E. coli cells.
Cell migration is critical to many important biological processes, such as cancer metastasis, wound healing, and so on. Understanding what’s going on in individual moving cells remains an attractive problem in the world of biological physics. Previous experimental and theoretical researches have clarified quite a few details in different aspects of moving cells, such as intracellular hydrodynamics, cytoskeleton dynamics, and etc. However, a model considering all such factors and cell morphology is not often used. Thanks to a phase field method, we are now able to implement such a comprehensive model. And in fact, such models have captured a lot of behavior of moving cells. My interest is to extend this phase field method to allow it to include membrane cellular dynamics and model the migration behavior of irregular-shaped individual amoeboid cells.
My current research is about the motility of Myxococcus xanthus bacteria. This Myxo bacteria are a flexible rod-shaped cells that move along long axis with periodic reversals. My research is focusing on simulating Myxo cells’ movements and how they form aggregates.